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| Raposeiras,Ruy; Marriel,Ivanildo Evódio; Muzzi,Maria Rita Scotti; Paiva,Edilson; Pereira Filho,Israel Alexandre; Carvalhais,Lilia Costa; Passos,Raul Vinícius Magalhães; Pinto,Patrícia Pereira; Sá,Nadja Maria Horta de. |
| The objective of this work was to identify the most competitive and effective Rhizobium strains in order to increase common bean yield by nitrogen fixation as alternative or complementation to the nitrogen fertilization. Competitiveness tests were lead in axenic conditions, in Cerrado soil pots and in three field experiments, with native Rhizobium strains that were previously identified, according to their effectiveness and genetic variability. The identification of strains in nodules was performed using serological tests (axenic conditions) - agglutination and enzyme linked immunosorbent (Elisa) assays - and random amplified polymorfic DNA (RAPD) (Cerrado soil). Plant yield was determined using the dry weight (greenhouse conditions), total N and grain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Nodule occupancy; Elisa; RAPD. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000300010 |
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